Determination of plasma fatty acid composition in neonates by gas chromatography
EM Bondia, AI Castellote, MC Lopez… - Journal of Chromatography …, 1994 - Elsevier
Total fatty acids in plasma of neonates have been analysed as their methyl esters by gas
chromatography. They were separated on a capillary column coated with a SP-2380
stationary phase. As little as 100 μl of plasma is used for the analysis. The extraction
procedure was performed with dichloromethane—methanol (2: 1) and fatty acids were
methylated with boron trifluoride—methanol. The quantification of fatty acids is based on an
internal standard method. Absolute values (μg fatty acid per 100 μl plasma) are given …
chromatography. They were separated on a capillary column coated with a SP-2380
stationary phase. As little as 100 μl of plasma is used for the analysis. The extraction
procedure was performed with dichloromethane—methanol (2: 1) and fatty acids were
methylated with boron trifluoride—methanol. The quantification of fatty acids is based on an
internal standard method. Absolute values (μg fatty acid per 100 μl plasma) are given …