[4] Isolation and use of liver cells
P Moldéus, J Högberg, S Orrenius - Methods in enzymology, 1978 - Elsevier
Publisher Summary This chapter describes the isolation and use of liver cells. The technique
is based on liver perfusion with collagenase after removal of Ca 2+ by preperfusion with a
chelator. Moderately high yields of viable, single hepatocytes with a smooth, spherical
appearance and essentially free of nonparenchymal cells are obtained. Early attempts to
isolate hepatocytes employed mechanical force and, subsequently, perfusion of the liver
with Ca 2+-or K+-chelators but were unsuccessful in obtaining viable cells in high yields …
is based on liver perfusion with collagenase after removal of Ca 2+ by preperfusion with a
chelator. Moderately high yields of viable, single hepatocytes with a smooth, spherical
appearance and essentially free of nonparenchymal cells are obtained. Early attempts to
isolate hepatocytes employed mechanical force and, subsequently, perfusion of the liver
with Ca 2+-or K+-chelators but were unsuccessful in obtaining viable cells in high yields …