[HTML][HTML] A molecular basis for integrin αMβ2 ligand binding promiscuity
VP Yakubenko, VK Lishko, SCT Lam… - Journal of Biological …, 2002 - ASBMB
The leukocyte integrin α M β 2 is a highly promiscuous leukocyte receptor capable of
binding a multitude of unrelated ligands. To understand the molecular basis for the broad
ligand recognition of α M β 2, the inter-integrin chimera was created. In the chimeric integrin,
the βd-α5 loop-α5 helix segment comprised of residues Lys 245–Arg 261 from the α MI
domain of α M β 2 was inserted into the framework of α L β 2. The construct was expressed
in HEK 293 cells, and the ability of generated cells to adhere to fibrinogen and its derivatives …
binding a multitude of unrelated ligands. To understand the molecular basis for the broad
ligand recognition of α M β 2, the inter-integrin chimera was created. In the chimeric integrin,
the βd-α5 loop-α5 helix segment comprised of residues Lys 245–Arg 261 from the α MI
domain of α M β 2 was inserted into the framework of α L β 2. The construct was expressed
in HEK 293 cells, and the ability of generated cells to adhere to fibrinogen and its derivatives …