[HTML][HTML] Identification and reconstruction of the binding site within αMβ2 for a specific and high affinity ligand, NIF

L Zhang, EF Plow - Journal of Biological Chemistry, 1997 - ASBMB
Engagement of the α M β 2 (CD11b/CD18, Mac-1) integrin on neutrophils supports adhesion
and induces various cellular responses. These responses can be blocked by a specific
ligand of α M β 2, neutrophil inhibitory factor (NIF). The molecular basis of α M β 2-NIF
interactions was studied. The single chain α M subunit, expressed on the surface of human
293 cells, bound NIF with an affinity equivalent to that of α M β 2 heterodimer. This
observation, coupled with previous data showing that the α MI domain alone supported high …