[CITATION][C] [23] Membrane-associated metalloproteinase recognized by characteristic cleavage of myelin basic protein: Assay and isolation
L Howard, P Glynn - Methods in enzymology, 1995 - Elsevier
Artifactual proteolysis following tissue homogenization and cell breakage is a common
problem in biochemistry. However, it was the observation of a pattern of protein degradation
likely to reflect an in vitro artifact that serendipitously allowed the identification of a novel
metalloproteinase in brain myelin membrane preparations. l. 2 This proteinase,
subsequently demonstrated in a variety of tissues, has been cloned and shown to be a
member of a newly recognized family of mammalian proteins related to the disintegrin …
problem in biochemistry. However, it was the observation of a pattern of protein degradation
likely to reflect an in vitro artifact that serendipitously allowed the identification of a novel
metalloproteinase in brain myelin membrane preparations. l. 2 This proteinase,
subsequently demonstrated in a variety of tissues, has been cloned and shown to be a
member of a newly recognized family of mammalian proteins related to the disintegrin …