Direct evidence for neurogenic inflammation and its prevention by denervation and by pretreatment with capsaicin.

N Jancso, A Jancsó-Gábor… - British journal of …, 1967 - ncbi.nlm.nih.gov
N Jancso, A Jancsó-Gábor, J Szolcsanyi
British journal of pharmacology and chemotherapy, 1967ncbi.nlm.nih.gov
METHODS Methods for the evaluation of theinflammatory reactions in rats Two methods
were used. In one of them we determined the vascular permeability quantitatively. For this
purpose the well-known Evans blue test was combined with an extraction technique
elaborated in our laboratory. The-dye which escapes from the vascular system in the course
of the inflammatory reaction was extracted from the tissues with methanol containing 1%
suramin (Bayer 205). Extraction was complete in about 2 weeks and the quantity of the …
METHODS Methods for the evaluation of theinflammatory reactions in rats Two methods were used. In one of them we determined the vascular permeability quantitatively. For this purpose the well-known Evans blue test was combined with an extraction technique elaborated in our laboratory. The-dye which escapes from the vascular system in the course of the inflammatory reaction was extracted from the tissues with methanol containing 1% suramin (Bayer 205). Extraction was complete in about 2 weeks and the quantity of the extracted dye was determined calorimetrically (Jancso-Gdbor, Szolcsdnyi & Jancs6, 1967). By the other method the localization of the inflammatory reaction was rendered visible in histological preparations (Jancs6, 1960, 1961). Immediately before applying the inflammatory stimuli, a 1% colloidal silver solution was injected into the tail vein of the rat (10 mg/100 g). Thereafter, the parts of the body involved in the inflammation process turned brown. Whereas Evans blue and other dyes used in inflammation research produce only diffuse patches of colour, with colloidal silver a good microscopic picture can be obtained which reveals the finest details of localization. In both methods the skin and mucous membranes were removed after killing the animals by bleeding.
Stimulation of the saphenous and trigeminal nerves in rats The effect of antidromic stimulation of the saphenous and trigeminal nerves was investigated. Under pentobarbitone anaesthesia (40 mg/kg) the saphenous nerve was exposed and cut high in the thigh. The peripheral end was stimulated with rectangular pulses by means of a bipolar platinum electrode. A wet chamber was used to avoid drying of the nerve. Animals were kept on a warmed plate and, when needed, an infra-red lamp was used so that the ambient temperatures could be kept at 36'C. In the case of the trigeminal nerve, its ophthalmic branch, and sometimes the maxillary branch too, were stimulated intracranially by means of a stereotaxic apparatus. The stimulating electrode was bipolar and consisted of two sharp stainless steel needles with a diameter of 0.5 mm placed with their tips 1.9 mm apart. They were insulated except for about 1.5 mm at their tips. Under pentobarbitone or buthalitone anaesthesia the top of the skull was trepanned and through the hole the bipolar electrode was inserted vertically so that the nerve was situated between the tips of the needles. The coordinates of the top of the electrode were: posterior= 1-2.5, lateral= 2-2.2, vertical= 11-12.5 mm related to the centre of intersection of the sagittal and coronary sutures. The nerve was stimulated for 20 min by rectangular pulses, in most cases with a voltage of 3 V, a duration of 0.8 msec and a frequency of 200/sec. Animals were kept warm by means of an infra-red lamp. After each experiment the skull was opened and the location of the electrodes was established.
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