SSX: A multigene family with several members transcribed in normal testis and human cancer

AO Gure, Ö Türeci, U Sahin, S Tsang… - … journal of cancer, 1997 - Wiley Online Library
AO Gure, Ö Türeci, U Sahin, S Tsang, MJ Scanlan, E Jäger, A Knuth, M Pfreundschuh…
International journal of cancer, 1997Wiley Online Library
Abstract Analysis of t (X; 18) translocation in synovial sarcoma had previously led to the
definition of the SSX2 gene, the fusion partner on chromosome X. Subsequent screening of
testicular cDNA libraries identified 2 highly homologous genes, SSX1 and SSX3. Among
these 3 genes, SSX2 has been found to be identical to HOM‐MEL‐40, which codes for an
immunogenic tumor antigen expressed in various human cancers. SSX2 thus belongs to the
family of cancer/testis (CT) antigens, ie, immunogenic protein antigens with characteristic …
Abstract
Analysis of t(X;18) translocation in synovial sarcoma had previously led to the definition of the SSX2 gene, the fusion partner on chromosome X. Subsequent screening of testicular cDNA libraries identified 2 highly homologous genes, SSX1 and SSX3. Among these 3 genes, SSX2 has been found to be identical to HOM‐MEL‐40, which codes for an immunogenic tumor antigen expressed in various human cancers. SSX2 thus belongs to the family of cancer/testis (CT) antigens, i.e., immunogenic protein antigens with characteristic mRNA expression in normal testis and in cancer. To define additional CT antigens, we have immuno‐screened a testicular cDNA expression library with an allogeneic serum from a melanoma patient, and both SSX2 and SSX3 were isolated. Further studies using testicular cDNA and SSX probes defined 2 new members of this gene family, SSX4 and SSX5, while a shorter cDNA variant of SSX4 was also identified. All 5 members of the SSX family shared strong sequence homology, with nucleotide homology ranging from 88 to 95% and amino acid homology ranging from 77 to 91%. Genomic cloning of a prototype SSX gene (SSX2) showed that its coding region is encoded by 6 exons, and the shortened form of SSX4 cDNA represents an alternatively spliced product lacking the 5th exon. Analysis of SSX mRNA expression by gene‐specific RT‐PCR confirmed that all 5 SSX genes are expressed in testis. In addition, analysis of a panel of 12 melanoma cell lines showed strong mRNA expression of either SSX1 (3/12), SSX2 (3/12), SSX4 (1/12), or SSX5 (1/12), indicating variable activation of the genes in malignant cells. Int. J. Cancer 72:965–971, 1997. © 1997 Wiley‐Liss, Inc.
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