Tests for autoimmune disease in otology
BF McCabe, KJ McCormick - Otology & Neurotology, 1984 - journals.lww.com
BF McCabe, KJ McCormick
Otology & Neurotology, 1984•journals.lww.comPrepare Sterilin leukocyte kk late with tissue culture surface by applyin silicone grease to the
top rim of each we to be used and a dot of silicone grease toward the lower center of the well
to hold the cut micropipette in place. After centrifugation, cut Crítoseal end within 2 to 4 mm
from cell-media interface (length cut dependent on packed cell length). Cut approximately I.
0 mm into packed cells and place into well on to of silicone grease. Immediately add antigen
suspension and media to a total vol-ume of 430A/well. Cover with a 22x22-mm coverslip that …
top rim of each we to be used and a dot of silicone grease toward the lower center of the well
to hold the cut micropipette in place. After centrifugation, cut Crítoseal end within 2 to 4 mm
from cell-media interface (length cut dependent on packed cell length). Cut approximately I.
0 mm into packed cells and place into well on to of silicone grease. Immediately add antigen
suspension and media to a total vol-ume of 430A/well. Cover with a 22x22-mm coverslip that …
Prepare Sterilin leukocyte kk late with tissue culture surface by applyin silicone grease to the top rim of each we to be used and a dot of silicone grease toward the lower center of the well to hold the cut micropipette in place. After centrifugation, cut Crítoseal end within 2 to 4 mm from cell-media interface (length cut dependent on packed cell length). Cut approximately I. 0 mm into packed cells and place into well on to of silicone grease. Immediately add antigen suspension and media to a total vol-ume of 430A/well.
Cover with a 22x22-mm coverslip that has been presoaked in 70% ethanol and flamed. Do not allow air bubbles to be left underneath coverslip. Repeat steps 10 and ll for each preparation to be tested. Completed test includes assays with: 1. no antigen, 2. Phytohemagglutinin (PHA)(2g/ml, final concentration), 3. inner ear membrane antigen (approximately= 5 pg/ml, final concen-tration), and 4. control antigen (approximately s5 pg/ml, final concentration). Each test is run in triplicate or quadruplicate,
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