[HTML][HTML] Contribution of the ERK5/MEK5 pathway to Ras/Raf signaling and growth control

JM English, G Pearson, T Hockenberry… - Journal of Biological …, 1999 - ASBMB
The activity of the catalytic domain of the orphan MAP kinase ERK5 is increased by Ras but
not Raf-1 in cells, which suggests that ERK5 might mediate Raf-independent signaling by
Ras. We found that Raf-1 does contribute to Ras activation of ERK5 but in a manner that
does not correlate with Raf-1 catalytic activity. A clue to the mechanism of action of Raf-1 on
ERK5 comes from the observation that endogenous Raf-1 binds to endogenous ERK5,
suggesting the involvement of regulatory protein-protein interactions. This interaction is …