[HTML][HTML] Components of a new human protein kinase signal transduction pathway

G Zhou, ZQ Bao, JE Dixon - Journal of Biological Chemistry, 1995 - ASBMB
We have identified two components of a new protein kinase signaling cascade, MAPK/ERK
kinase 5 (MEK5) and extracellular signal-regulated kinase 5 (ERK5). The MEK5 cDNA was
isolated by degenerate PCR and encodes a 444-amino acid protein, which has
approximately 40% identity to known MEKs. ERK5 was identified by a specific interaction
with the MEK5 mutants S311A/T315A and K195M in the yeast two-hybrid system. The
proteins were found to interact in an in vitro binding assay as well. ERK5 did not interact with …