Synthesis of pyrene derivatives of cerebroside sulfate and their use for determining arylsulfatase A activity
S Marchesini, P Viani, B Cestaro, S Gatt - Biochimica et Biophysica Acta …, 1989 - Elsevier
Two fluorescent derivatives of cerebroside sulfate ('sulfatide') have been synthesized and
used as substrates for determining arylsulfatase A activity. These were 12-(1-pyrene)
dodecanoyl cerebroside sulfate (P12-sulfatide) and 12 (1-pyrenesulfonylamido) dodecanoyl
cerebroside sulfate (PSA12-sulfatide). When incubated at pH 5.0 in the presence of 5 mM
MnCl 2 and 5.5 mM of taurodeoxycholate, either substrate was hydrolyzed by arylsulfatase A
of human leukocytes. The rate of hydrolysis was proportional to the incubation time and …
used as substrates for determining arylsulfatase A activity. These were 12-(1-pyrene)
dodecanoyl cerebroside sulfate (P12-sulfatide) and 12 (1-pyrenesulfonylamido) dodecanoyl
cerebroside sulfate (PSA12-sulfatide). When incubated at pH 5.0 in the presence of 5 mM
MnCl 2 and 5.5 mM of taurodeoxycholate, either substrate was hydrolyzed by arylsulfatase A
of human leukocytes. The rate of hydrolysis was proportional to the incubation time and …