[HTML][HTML] Activation of the leukocyte NADPH oxidase by phorbol ester requires the phosphorylation of p47PHOX on serine 303 or 304
O Inanami, JL Johnson, JK McAdara, J El Benna… - Journal of Biological …, 1998 - ASBMB
The leukocyte NADPH oxidase is an enzyme in phagocytes and B lymphocytes that when
activated catalyzes the production of O· ̄ 2 from oxygen and NADPH. During oxidase
activation, serine residues in the C-terminal quarter of the oxidase component p47 PHOX
become extensively phosphorylated, the protein acquiring as many as 9 phosphate
residues. In a study of 11 p47 PHOX mutants, each containing an alanine instead of a serine
at a single potential phosphorylation site, we found that all but S379A corrected the defect in …
activated catalyzes the production of O· ̄ 2 from oxygen and NADPH. During oxidase
activation, serine residues in the C-terminal quarter of the oxidase component p47 PHOX
become extensively phosphorylated, the protein acquiring as many as 9 phosphate
residues. In a study of 11 p47 PHOX mutants, each containing an alanine instead of a serine
at a single potential phosphorylation site, we found that all but S379A corrected the defect in …