[HTML][HTML] The β Subunit of the High Conductance Calcium-activated Potassium Channel: IDENTIFICATION OF RESIDUES INVOLVED IN CHARYBDOTOXIN BINDING
M Hanner, R Vianna-Jorge, A Kamassah… - Journal of Biological …, 1998 - ASBMB
Coexpression of α and β subunits of the high conductance Ca 2+-activated K+(maxi-K)
channel leads to a 50-fold increase in the affinity for 125 I-charybdotoxin (125 I-ChTX) as
compared with when the α subunit is expressed alone (Hanner, M., Schmalhofer, WA,
Munujos, P., Knaus, H.-G., Kaczorowski, GJ, and Garcia, ML (1997) Proc. Natl. Acad. Sci.
USA 94, 2853–2858). To identify those residues in the β subunit that are responsible for this
change in binding affinity, Ala scanning mutagenesis was carried out along the extracellular …
channel leads to a 50-fold increase in the affinity for 125 I-charybdotoxin (125 I-ChTX) as
compared with when the α subunit is expressed alone (Hanner, M., Schmalhofer, WA,
Munujos, P., Knaus, H.-G., Kaczorowski, GJ, and Garcia, ML (1997) Proc. Natl. Acad. Sci.
USA 94, 2853–2858). To identify those residues in the β subunit that are responsible for this
change in binding affinity, Ala scanning mutagenesis was carried out along the extracellular …