Human CD8+ TCR-αβ+ and TCR-γδ+ cells modulate autologous autoreactive neuroantigen-specific CD4+ T-cells by different mechanisms
J Correale, M Rojany, LP Weiner - Journal of neuroimmunology, 1997 - Elsevier
To investigate the regulatory interactions among autologous T-cells during the course of
multiple sclerosis (MS), proteolipid protein peptide-specific CD4+ T-cell clones (TCCs) were
irradiated and used as immunogens to stimulate purified populations of autologous CD8+
TCR-αβ+ and TCR-γδ+ T-cells isolated from the peripheral blood of MS patients, patients
with other non-inflammatory neurological diseases, and healthy blood donors. The resulting
blasts were expanded in the presence of hIL-2 and then cloned by limiting dilution. Two …
multiple sclerosis (MS), proteolipid protein peptide-specific CD4+ T-cell clones (TCCs) were
irradiated and used as immunogens to stimulate purified populations of autologous CD8+
TCR-αβ+ and TCR-γδ+ T-cells isolated from the peripheral blood of MS patients, patients
with other non-inflammatory neurological diseases, and healthy blood donors. The resulting
blasts were expanded in the presence of hIL-2 and then cloned by limiting dilution. Two …