Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.
Proceedings of the National Academy of Sciences, 1986•National Acad Sciences
This report describes the use of fluorescence in situ hybridization for chromosome
classification and detection of chromosome aberrations. Biotin-labeled DNA was hybridized
to target chromosomes and subsequently rendered fluorescent by successive treatments
with fluorescein-labeled avidin and biotinylated anti-avidin antibody. Human chromosomes
in human-hamster hybrid cell lines were intensely and uniformly stained in metaphase
spreads and interphase nuclei when human genomic DNA was used as a probe …
classification and detection of chromosome aberrations. Biotin-labeled DNA was hybridized
to target chromosomes and subsequently rendered fluorescent by successive treatments
with fluorescein-labeled avidin and biotinylated anti-avidin antibody. Human chromosomes
in human-hamster hybrid cell lines were intensely and uniformly stained in metaphase
spreads and interphase nuclei when human genomic DNA was used as a probe …
This report describes the use of fluorescence in situ hybridization for chromosome classification and detection of chromosome aberrations. Biotin-labeled DNA was hybridized to target chromosomes and subsequently rendered fluorescent by successive treatments with fluorescein-labeled avidin and biotinylated anti-avidin antibody. Human chromosomes in human-hamster hybrid cell lines were intensely and uniformly stained in metaphase spreads and interphase nuclei when human genomic DNA was used as a probe. Interspecies translocations were detected easily at metaphase. The human-specific fluorescence intensity from cell nuclei and chromosomes was proportional to the amount of target human DNA. Human Y chromosomes were fluorescently stained in metaphase and interphase nuclei by using a 0.8-kilobase DNA probe specific for the Y chromosome. Cells from males were 40 times brighter than those from females. Both Y chromosomal domains were visible in most interphase nuclei of XYY amniocytes. Human 28S ribosomal RNA genes on metaphase chromosomes were distinctly stained by using a 1.5-kilobase DNA probe.
National Acad Sciences