[HTML][HTML] Cloning, expression, and functional characterization of the β regulatory subunit of human methionine adenosyltransferase (MAT II)
HL LeGros, AB Halim, AM Geller, M Kotb - Journal of Biological Chemistry, 2000 - ASBMB
MAT II, the extrahepatic form of methionine adenosyltransferase (MAT), consists of catalytic
α 2/α 2′ subunits and a noncatalytic β subunit, believed to have a regulatory function. The
full-length cDNA that encodes the β subunit of human MAT II was cloned and found to
encode for a 334-amino acid protein with a calculated molecular weight of 37,552. Analysis
of sequence homology showed similarity with bacterial enzymes that catalyze the reduction
of TDP-linked sugars. The β subunit cDNA was cloned into the pQE-30 expression vector …
α 2/α 2′ subunits and a noncatalytic β subunit, believed to have a regulatory function. The
full-length cDNA that encodes the β subunit of human MAT II was cloned and found to
encode for a 334-amino acid protein with a calculated molecular weight of 37,552. Analysis
of sequence homology showed similarity with bacterial enzymes that catalyze the reduction
of TDP-linked sugars. The β subunit cDNA was cloned into the pQE-30 expression vector …