A quick, easy and inexpensive method for the isolation of human peripheral blood monocytes
GM Graziani-Bowering, JM Graham… - Journal of immunological …, 1997 - Elsevier
A commercial monocyte isolation technique based on the OptiPrep density-gradient medium
was up-scaled with respect to sample and reagent volumes. The results of 7 isolations are
reported in which the average purity ranged from 87.9 to 96.4%. In all but the initial isolation,
monocytes were defined as CD15+ dim CD4+ dim as assessed by flow cytometric analysis;
in the first isolation, monocytes were defined by the traditional CD14+ CD4+ dim
combination. The mean yield (the number of isolated monocytes relative to the number …
was up-scaled with respect to sample and reagent volumes. The results of 7 isolations are
reported in which the average purity ranged from 87.9 to 96.4%. In all but the initial isolation,
monocytes were defined as CD15+ dim CD4+ dim as assessed by flow cytometric analysis;
in the first isolation, monocytes were defined by the traditional CD14+ CD4+ dim
combination. The mean yield (the number of isolated monocytes relative to the number …