[HTML][HTML] Hydrogen peroxide activates NF-κB through tyrosine phosphorylation of IκBα and serine phosphorylation of p65: evidence for the involvement of IκBα kinase …
Y Takada, A Mukhopadhyay, GC Kundu… - Journal of Biological …, 2003 - ASBMB
Although it is well established that reactive oxygen intermediates mediate the NF-κB
activation induced by most agents, how H 2 O 2 activates this transcription factor is not well
understood. We found that treatment of human myeloid KBM-5 cells with H 2 O 2 activated
NF-κB in a dose-and time-dependent manner much as tumor necrosis factor (TNF) did but
unlike TNF, H 2 O 2 had no effect on IκBα degradation. Unexpectedly, however, like TNF-
induced activation, H 2 O 2-induced NF-κB activation was blocked by the calpain inhibitor N …
activation induced by most agents, how H 2 O 2 activates this transcription factor is not well
understood. We found that treatment of human myeloid KBM-5 cells with H 2 O 2 activated
NF-κB in a dose-and time-dependent manner much as tumor necrosis factor (TNF) did but
unlike TNF, H 2 O 2 had no effect on IκBα degradation. Unexpectedly, however, like TNF-
induced activation, H 2 O 2-induced NF-κB activation was blocked by the calpain inhibitor N …