Molecular cloning of a ligand for the flt3flk-2 tyrosine kinase receptor: A proliferative factor for primitive hematopoietic cells

SD Lyman, L James, TV Bos, P de Vries, K Brasel… - Cell, 1993 - cell.com
SD Lyman, L James, TV Bos, P de Vries, K Brasel, B Gliniak, LT Hollingsworth, KS Picha…
Cell, 1993cell.com
Cloning of a ligand for the murine flt3/flk-2 tyrosine kinase receptor was undertaken using a
soluble form of the receptor to identify a source of ligand. A murine T cell line, P750. 3A4,
was identified that appeared to express a cell surface ligand for this receptor. A cDNA clone
was isolated from an expression library prepared from these cells that was capable, when
transfer&d into cells, of conferring binding to a soluble form of the flt% flk-2 receptor. The
cDNA for this ligand encodes a type I transmembrane protein that stimulates the proliferation …
Summary
Cloning of a ligand for the murine flt3/flk-2 tyrosine kinase receptor was undertaken using a soluble form of the receptor to identify a source of ligand. A murine T cell line, P750. 3A4, was identified that appeared to express a cell surface ligand for this receptor. A cDNA clone was isolated from an expression library prepared from these cells that was capable, when transfer&d into cells, of conferring binding to a soluble form of the flt% flk-2 receptor. The cDNA for this ligand encodes a type I transmembrane protein that stimulates the proliferation of cells transfected with the flU/flk-2 recep tor. A soluble form of the ligand stimulates the proliferation of deflned subpopulations of murine bone marrow and fetal liver cells as well as human bone marrow cells that are highly enriched for hematopoietic stem cells and primitive uncommitted progenitor cells.
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