[HTML][HTML] Transcriptional cross-talk between Smad, ERK1/2, and p38 mitogen-activated protein kinase pathways regulates transforming growth factor-β-induced …
H Watanabe, MP de Caestecker, Y Yamada - Journal of Biological …, 2001 - ASBMB
In chondrogenesis, members of the transforming growth factor-β (TGF-β) superfamily play
critical roles by inducing gene expression of cartilage-specific molecules. By using a
chondrogenic cell line, ATDC5, we investigated the TGF-β-mediated signaling pathways
involved in expression of the aggrecan gene (Agc). At confluency, TGF-β induced
Agcexpression within 3 h, and cycloheximide blocked this induction, indicating that de novo
protein synthesis is essential for this response. At this stage, TGF-β induced rapid, transient …
critical roles by inducing gene expression of cartilage-specific molecules. By using a
chondrogenic cell line, ATDC5, we investigated the TGF-β-mediated signaling pathways
involved in expression of the aggrecan gene (Agc). At confluency, TGF-β induced
Agcexpression within 3 h, and cycloheximide blocked this induction, indicating that de novo
protein synthesis is essential for this response. At this stage, TGF-β induced rapid, transient …