Human endothelial cells: use of heparin in cloning and long-term serial cultivation
SC Thornton, SN Mueller, EM Levine - Science, 1983 - science.org
SC Thornton, SN Mueller, EM Levine
Science, 1983•science.orgEndothelial cells from human blood vessels were cultured in vitro, with doubling times of 17
to 21 hours for 42 to 79 population doublings. Cloned human endothelial cell strains were
established for the first time and had similar proliferative capacities. This vigorous cell
growth was achieved by addition of heparin to culture medium containing reduced
concentrations of endothelial cell growth factor. The routine cloning and long-term culture of
human endothelial cells will facilitate studying the human endothelium in vitro.
to 21 hours for 42 to 79 population doublings. Cloned human endothelial cell strains were
established for the first time and had similar proliferative capacities. This vigorous cell
growth was achieved by addition of heparin to culture medium containing reduced
concentrations of endothelial cell growth factor. The routine cloning and long-term culture of
human endothelial cells will facilitate studying the human endothelium in vitro.
Endothelial cells from human blood vessels were cultured in vitro, with doubling times of 17 to 21 hours for 42 to 79 population doublings. Cloned human endothelial cell strains were established for the first time and had similar proliferative capacities. This vigorous cell growth was achieved by addition of heparin to culture medium containing reduced concentrations of endothelial cell growth factor. The routine cloning and long-term culture of human endothelial cells will facilitate studying the human endothelium in vitro.
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