[HTML][HTML] Targeted inactivation of the isoprenylcysteine carboxyl methyltransferase gene causes mislocalization of K-Ras in mammalian cells
MO Bergo, GK Leung, P Ambroziak, JC Otto… - Journal of Biological …, 2000 - ASBMB
After isoprenylation and endoproteolytic processing, the Ras proteins are methylated at the
carboxyl-terminal isoprenylcysteine. The importance of isoprenylation for targeting of Ras
proteins to the plasma membrane is well established, but the importance of carboxyl
methylation, which is carried out by isoprenylcysteine carboxyl methyltransferase (Icmt), is
less certain. We used gene targeting to produce homozygous Icmt knockout embryonic stem
cells (Icmt−/−). Lysates fromIcmt−/− cells lacked the ability to methylate farnesyl-K-Ras4B or …
carboxyl-terminal isoprenylcysteine. The importance of isoprenylation for targeting of Ras
proteins to the plasma membrane is well established, but the importance of carboxyl
methylation, which is carried out by isoprenylcysteine carboxyl methyltransferase (Icmt), is
less certain. We used gene targeting to produce homozygous Icmt knockout embryonic stem
cells (Icmt−/−). Lysates fromIcmt−/− cells lacked the ability to methylate farnesyl-K-Ras4B or …