Comparison of different DNA preparation protocols for PCR diagnosis of Human African Trypanosomosis in Cote d'Ivoire
P Solano, V Jamonneau, P N'guessan, L N'dri, NN Dje… - Acta tropica, 2002 - Elsevier
During a medical survey the sleeping sickness focus in Bonon, Ivory Coast, PCR with
Trypanosoma brucei specific primers (TBR 1–2 from Parasitology 99 (1989) 57) was tested
on DNA derived from blood samples. DNA purification using a chelating resin was
performed either on whole blood or on the buffy coat prepared in two different ways. The
preparation based on whole blood performed better than those using the buffy-coat. Using
this first method, the sensitivity was 100% on parasitologically confirmed patients, and the …
Trypanosoma brucei specific primers (TBR 1–2 from Parasitology 99 (1989) 57) was tested
on DNA derived from blood samples. DNA purification using a chelating resin was
performed either on whole blood or on the buffy coat prepared in two different ways. The
preparation based on whole blood performed better than those using the buffy-coat. Using
this first method, the sensitivity was 100% on parasitologically confirmed patients, and the …