[HTML][HTML] Effects of nitroglycerin on soluble guanylate cyclase: implications for nitrate tolerance
JD Artz, B Schmidt, JL McCracken… - Journal of Biological …, 2002 - ASBMB
Soluble guanylate cyclase (sGC) is a heterodimeric hemoprotein that catalyzes the
conversion of GTP to cGMP. Upon binding NO to its heme cofactor, purified sGC was
activated 300-fold. sGC was only activated 67-fold by nitroglycerin (GTN) and Cys; and in
the absence of Cys, GTN did not activate sGC. Electronic absorption spectroscopy studies
showed that upon NO binding, the Soret of ferrous sGC shifted from 431 to 399 nm. The data
also revealed that activation of sGC by GTN/Cys was not via the expected ferrous heme-NO …
conversion of GTP to cGMP. Upon binding NO to its heme cofactor, purified sGC was
activated 300-fold. sGC was only activated 67-fold by nitroglycerin (GTN) and Cys; and in
the absence of Cys, GTN did not activate sGC. Electronic absorption spectroscopy studies
showed that upon NO binding, the Soret of ferrous sGC shifted from 431 to 399 nm. The data
also revealed that activation of sGC by GTN/Cys was not via the expected ferrous heme-NO …