A new sensitive chemiluminescence probe, L-012, for measuring the production of superoxide anion by cells

Y Nishinaka, Y Aramaki, H Yoshida, H Masuya… - Biochemical and …, 1993 - Elsevier
Y Nishinaka, Y Aramaki, H Yoshida, H Masuya, T Sugawara, Y Ichimori
Biochemical and biophysical research communications, 1993Elsevier
A sensitive chemiluminescence method for measuring the production of superoxide anion
(O 2−) by activated EoL-1 cells (human eosinophilic leukemia cell line) is described.
Recently, we succeeded in synthesizing a new chemiluminescence probe, 8-amino-5-chloro-
7-phenylpyrido [3, 4-d] pyridazine-1, 4 (2H, 3H) dione (L-012). In the presence of L-012,
activated EoL-1 cells which produce reactive oxygen species generated a marked
chemiluminescence with negligible background. The L-012-dependent chemiluminescence …
Abstract
A sensitive chemiluminescence method for measuring the production of superoxide anion (O2) by activated EoL-1 cells (human eosinophilic leukemia cell line) is described. Recently, we succeeded in synthesizing a new chemiluminescence probe, 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine- 1 ,4(2H,3H)dione (L-012). In the presence of L-012, activated EoL-1 cells which produce reactive oxygen species generated a marked chemiluminescence with negligible background. The L-012-dependent chemiluminescence was completely abolished by 100-300 U/ml superoxide dismutase, indicating that the main reactive oxygen species detected in this reaction was O2. The light intensity and the sensitivity of L-012 to O2 were higher than those of other chemiluminescence probes such as luminol and Cypridina luciferin analog (MCLA). Thus, L-012 would provide an improved chemiluminescence method for measuring O2 from cells.
Elsevier