Isolation of a novel siderophore from Pseudomonas cepacia

PA Sokol, CJ Lewis, JJ Dennis - Journal of medical …, 1992 - microbiologyresearch.org
PA Sokol, CJ Lewis, JJ Dennis
Journal of medical microbiology, 1992microbiologyresearch.org
A novel iron-binding compound was identified in ethyl acetate extracts of the supernates
from Pseudomonas cepacia cultures. This compound, named azurechelin, was produced by
88% of P. cepacia strains isolated from the respiratory tract. Production of azurechelin was
regulated by the iron concentration in the culture medium. Azurechelin enhanced the growth
of P. cepacia in a medium containing transferrin 200 mg/L. Azurechelin released iron from
transferrin in an equilibrium dialysis assay, suggesting that it could compete with transferrin …
Summary
A novel iron-binding compound was identified in ethyl acetate extracts of the supernates from Pseudomonas cepacia cultures. This compound, named azurechelin, was produced by 88% of P. cepacia strains isolated from the respiratory tract. Production of azurechelin was regulated by the iron concentration in the culture medium. Azurechelin enhanced the growth of P. cepacia in a medium containing transferrin 200 mg/L. Azurechelin released iron from transferrin in an equilibrium dialysis assay, suggesting that it could compete with transferrin for iron. Azurechelin could also stimulate iron uptake by P. cepacia. This siderophore appeared to have a novel structure with neither the typical characteristics of catechol nor of hydroxamate compounds.
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