The analysis of diagnostic markers of genetic disorders in human blood and urine using tandem mass spectrometry with liquid secondary ion mass spectrometry
DS Millington, N Kodo, N Terada, D Roe… - International Journal of …, 1991 - Elsevier
A method has been developed for the rapid diagnosis of metabolic diseases based on the
analysis of characteristic metabolites in body fluids by fast atom bombardment or liquid
secondary ion tandem mass spectrometry (FAB-MS—MS or LSIMS—MS). Acylcarnitine
profiles were obtained from 100 μl urine. 200 μl plasma or 25 μl whole blood spotted onto
filter paper by simple solvent extraction, esterification and analysis using a precursor ion
scan function on a triple quadrupole mass spectrometer. Specificity and sensitivity were …
analysis of characteristic metabolites in body fluids by fast atom bombardment or liquid
secondary ion tandem mass spectrometry (FAB-MS—MS or LSIMS—MS). Acylcarnitine
profiles were obtained from 100 μl urine. 200 μl plasma or 25 μl whole blood spotted onto
filter paper by simple solvent extraction, esterification and analysis using a precursor ion
scan function on a triple quadrupole mass spectrometer. Specificity and sensitivity were …