Neutral amino acid transport. Characterization of the A and L systems in isolated rat hepatocytes.
A Le Cam, P Freychet - Journal of Biological Chemistry, 1977 - ASBMB
Hepatocytes isolated from adult rat liver by enzymatic dispersion were used to investigate
amino acid transport. Steady state and influx experiments were carried out with alpha-amino
[1-14C] isobutyric acid and [1-14C] cycloleucine in the presence and absence of sodium
under various experimental conditions. Hepatocytes concentrated alpha-aminoisobutyric
acid to a 3-fold higher degree than cycloleucine. At low external alpha-aminoisobutyric acid
levels (2 to 5 mM), about 25% and 75% of entry were accounted for by nonsaturable and …
amino acid transport. Steady state and influx experiments were carried out with alpha-amino
[1-14C] isobutyric acid and [1-14C] cycloleucine in the presence and absence of sodium
under various experimental conditions. Hepatocytes concentrated alpha-aminoisobutyric
acid to a 3-fold higher degree than cycloleucine. At low external alpha-aminoisobutyric acid
levels (2 to 5 mM), about 25% and 75% of entry were accounted for by nonsaturable and …