Quantification of antigen specific CD8+ T cells using an ELISPOT assay
Y Miyahira, K Murata, D Rodriguez… - Journal of …, 1995 - Elsevier
An ELISPOT assay to detect and determine the number of antigen specific CD8+ T cells was
standardized using cloned murine CD8+ T cells specific for the epitope SYVPSAEQI of a
rodent malaria antigen. This assay is based on the detection of IFN-γ secretion by single
cells after their stimulation with antigen. The interferon secretion is visualized as spots
revealed by using enzyme labeled anti-IFN-γ monoclonal antibodies. Using known numbers
of cloned murine CD8+ T cells it was determined that the assay detects 80–95% of these …
standardized using cloned murine CD8+ T cells specific for the epitope SYVPSAEQI of a
rodent malaria antigen. This assay is based on the detection of IFN-γ secretion by single
cells after their stimulation with antigen. The interferon secretion is visualized as spots
revealed by using enzyme labeled anti-IFN-γ monoclonal antibodies. Using known numbers
of cloned murine CD8+ T cells it was determined that the assay detects 80–95% of these …