Tetraspanin CD151 regulates α6β1 integrin adhesion strengthening
J Lammerding, AR Kazarov, H Huang… - Proceedings of the …, 2003 - National Acad Sciences
J Lammerding, AR Kazarov, H Huang, RT Lee, ME Hemler
Proceedings of the National Academy of Sciences, 2003•National Acad SciencesThe tetraspanin CD151 molecule associates specifically with laminin-binding integrins,
including α6β1. To probe strength of α6β1-dependent adhesion to laminin-1, defined forces
(0–1.5 nN) were applied to magnetic laminin-coated microbeads bound to NIH 3T3 cells. For
NIH 3T3 cells bearing wild-type CD151, adhesion strengthening was observed, as bead
detachment became more difficult over time. In contrast, mutant CD151 (with the C-terminal
region replaced) showed impaired adhesion strengthening. Static cell adhesion to laminin …
including α6β1. To probe strength of α6β1-dependent adhesion to laminin-1, defined forces
(0–1.5 nN) were applied to magnetic laminin-coated microbeads bound to NIH 3T3 cells. For
NIH 3T3 cells bearing wild-type CD151, adhesion strengthening was observed, as bead
detachment became more difficult over time. In contrast, mutant CD151 (with the C-terminal
region replaced) showed impaired adhesion strengthening. Static cell adhesion to laminin …
The tetraspanin CD151 molecule associates specifically with laminin-binding integrins, including α6β1. To probe strength of α6β1-dependent adhesion to laminin-1, defined forces (0–1.5 nN) were applied to magnetic laminin-coated microbeads bound to NIH 3T3 cells. For NIH 3T3 cells bearing wild-type CD151, adhesion strengthening was observed, as bead detachment became more difficult over time. In contrast, mutant CD151 (with the C-terminal region replaced) showed impaired adhesion strengthening. Static cell adhesion to laminin-1, and detachment of beads coated with fibronectin or anti-α6 antibody were all unaffected by CD151 mutation. Hence, CD151 plays a key role in selectively strengthening α6β1 integrin-mediated adhesion to laminin-1.
National Acad Sciences