[HTML][HTML] Deletion Mutagenesis of Human Cystathionine β-Synthase: Impact on activity, oligomeric status, ands-adenosylmethionine regulation
J Oliveriusová, V Kery, KN Maclean, JP Kraus - Journal of Biological …, 2002 - ASBMB
Cystathionine β-synthase is a tetrameric hemeprotein that catalyzes the pyridoxal 5′-
phosphate-dependent condensation of serine and homocysteine to cystathionine. We have
used deletion mutagenesis of both the N and C termini to investigate the functional
organization of the catalytic and regulatory regions of this enzyme. Western blot analysis of
these mutants expressed in Escherichia coliindicated that residues 497–543 are involved in
tetramer formation. Deletion of the 70 N-terminal residues resulted in a heme-free protein …
phosphate-dependent condensation of serine and homocysteine to cystathionine. We have
used deletion mutagenesis of both the N and C termini to investigate the functional
organization of the catalytic and regulatory regions of this enzyme. Western blot analysis of
these mutants expressed in Escherichia coliindicated that residues 497–543 are involved in
tetramer formation. Deletion of the 70 N-terminal residues resulted in a heme-free protein …