Comparison of the promoters of the mouse (APEX) and human (APE) apurinic endonuclease genes
L Harrison, AG Ascione, Y Takiguchi… - Mutation Research/DNA …, 1997 - Elsevier
We investigated the minimal promoter of APEX, which encodes mouse apurinic DNA repair
endonuclease. A 1.85-kb fragment with APEX upstream sequences and∼ 290 bp of the
transcribed region linked to a chloramphenicol acetyltransferase (CAT) reporter gene was
assayed by transient transfection in NIH-3T3 cells. The minimal APEX promoter was
comprised of∼ 190 bp of upstream and∼ 170 bp of transcribed DNA (exon 1 and most of
intron 1). This∼ 360-bp region contains two CCAAT boxes and other consensus protein …
endonuclease. A 1.85-kb fragment with APEX upstream sequences and∼ 290 bp of the
transcribed region linked to a chloramphenicol acetyltransferase (CAT) reporter gene was
assayed by transient transfection in NIH-3T3 cells. The minimal APEX promoter was
comprised of∼ 190 bp of upstream and∼ 170 bp of transcribed DNA (exon 1 and most of
intron 1). This∼ 360-bp region contains two CCAAT boxes and other consensus protein …