The Notch ligand, Jagged-1, influences the development of primitive hematopoietic precursor cells

B Varnum-Finney, LE Purton, M Yu… - Blood, The Journal …, 1998 - ashpublications.org
B Varnum-Finney, LE Purton, M Yu, C Brashem-Stein, D Flowers, S Staats, KA Moore
Blood, The Journal of the American Society of Hematology, 1998ashpublications.org
We examined the expression of two members of the Notch family, Notch-1 and Notch-2, and
one Notch ligand, Jagged-1, in hematopoietic cells. Both Notch-1 and Notch-2 were
detected in murine marrow precursors (Lin− Sca-1+ c-kit+). The Notch ligand, Jagged-1, was
not detected in whole marrow or in precursors. However, Jagged-1 was seen in cultured
primary murine fetal liver stroma, cultured primary murine bone marrow stroma, and in
stromal cell lines. These results indicate a potential role for Notch-Notch ligand interactions …
Abstract
We examined the expression of two members of theNotch family, Notch-1 and Notch-2, and one Notch ligand, Jagged-1, in hematopoietic cells. Both Notch-1 and Notch-2 were detected in murine marrow precursors (LinSca-1+c-kit+). The Notch ligand, Jagged-1, was not detected in whole marrow or in precursors. However, Jagged-1 was seen in cultured primary murine fetal liver stroma, cultured primary murine bone marrow stroma, and in stromal cell lines. These results indicate a potential role for Notch-Notch ligand interactions in hematopoiesis. To further test this possibility, the effect of Jagged-1 on murine marrow precursor cells was assessed by coculturing sorted precursor cells (LinSca-1+c-kit+) with a 3T3 cell layer that expressed human Jagged-1 or by incubating sorted precursors with beads coated with the purified extracellular domain of human Jagged-1 (Jagged-1ext). We found that Jagged-1, presented both on the cell surface and on beads, promoted a twofold to threefold increase in the formation of primitive precursor cell populations. These results suggest a potential use for Notch ligands in expanding precursor cell populations in vitro.
ashpublications.org