The sae locus of Staphylococcus aureus encodes a two-component regulatory system
AT Giraudo, A Calzolari, AA Cataldi… - FEMS microbiology …, 1999 - academic.oup.com
AT Giraudo, A Calzolari, AA Cataldi, C Bogni, R Nagel
FEMS microbiology letters, 1999•academic.oup.comAbstract sae is a regulatory locus that activates the production of several exoproteins in
Staphylococcus aureus. A 3.4-kb fragment of a S. aureus genomic library, screened with a
probe adjacent to the transposon insertion of a sae:: Tn 551 mutant, was cloned into a
bifunctional vector. This fragment was shown to carry the sae locus by restoration of
exoprotein production in sae mutants. The sae locus was mapped to the Sma ID fragment of
the staphylococcal chromosome by pulse-field electrophoresis. Sequence analysis of the …
Staphylococcus aureus. A 3.4-kb fragment of a S. aureus genomic library, screened with a
probe adjacent to the transposon insertion of a sae:: Tn 551 mutant, was cloned into a
bifunctional vector. This fragment was shown to carry the sae locus by restoration of
exoprotein production in sae mutants. The sae locus was mapped to the Sma ID fragment of
the staphylococcal chromosome by pulse-field electrophoresis. Sequence analysis of the …
Abstract
sae is a regulatory locus that activates the production of several exoproteins in Staphylococcus aureus. A 3.4-kb fragment of a S. aureus genomic library, screened with a probe adjacent to the transposon insertion of a sae::Tn551 mutant, was cloned into a bifunctional vector. This fragment was shown to carry the sae locus by restoration of exoprotein production in sae mutants. The sae locus was mapped to the SmaI-D fragment of the staphylococcal chromosome by pulse-field electrophoresis. Sequence analysis of the cloned fragment revealed the presence of two genes, designated saeR and saeS, encoding a response regulator and a histidine protein kinase, respectively, with high homology to other bacterial two-component regulatory systems.
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