[HTML][HTML] Proprotein convertase PC1/3-related peptides are potent slow tight-binding inhibitors of murine PC1/3 and Hfurin
A Boudreault, D Gauthier, C Lazure - Journal of Biological Chemistry, 1998 - ASBMB
The proprotein convertase PC1/3 belongs to the subtilisin/kexin-like endoprotease family
and is synthesized as a preproenzyme. To investigate the function of its propeptide, murine
proPC1/3 and preproPC1/3 were isolated from the inclusion bodies of recombinant
preproPC1/3 baculovirus-infected insect cells, rendered soluble with 6 m guanidine HCl and
20 mmdithiothreitol, and purified by gel filtration and metal-binding affinity chromatography.
Two NH 2-terminal fragments containing the complete propeptide 1–84 region were …
and is synthesized as a preproenzyme. To investigate the function of its propeptide, murine
proPC1/3 and preproPC1/3 were isolated from the inclusion bodies of recombinant
preproPC1/3 baculovirus-infected insect cells, rendered soluble with 6 m guanidine HCl and
20 mmdithiothreitol, and purified by gel filtration and metal-binding affinity chromatography.
Two NH 2-terminal fragments containing the complete propeptide 1–84 region were …