Isolation and characterization of a novel secretory protein, stromal cell-derived factor-2 (SDF-2) using the signal sequence trap method
T Hamada, K Tashiro, H Tada, J Inazawa, M Shirozu… - Gene, 1996 - Elsevier
T Hamada, K Tashiro, H Tada, J Inazawa, M Shirozu, K Shibahara, T Nakamura, N Martina…
Gene, 1996•ElsevierWith use of the signal sequence trap method, we isolated a cDNA encoding a novel
secretory protein, SDF-2, from the mouse stromal cell line, ST2. The human homologue of
SDF-2 was also isolated. The amino acid (aa) sequences deduced from both the clones
were conserved more than 92%. The chromosomal localization of the human SDF-2 gene
was mapped to 17q11. 2. The aa sequence of SDF-2 shows similarity to those of yeast
dolichyl phosphate-d-mannose: protein mannosyltransferases, Pmtlp [Strahl-Bolsinger et …
secretory protein, SDF-2, from the mouse stromal cell line, ST2. The human homologue of
SDF-2 was also isolated. The amino acid (aa) sequences deduced from both the clones
were conserved more than 92%. The chromosomal localization of the human SDF-2 gene
was mapped to 17q11. 2. The aa sequence of SDF-2 shows similarity to those of yeast
dolichyl phosphate-d-mannose: protein mannosyltransferases, Pmtlp [Strahl-Bolsinger et …
With use of the signal sequence trap method, we isolated a cDNA encoding a novel secretory protein, SDF-2, from the mouse stromal cell line, ST2. The human homologue of SDF-2 was also isolated. The amino acid (aa) sequences deduced from both the clones were conserved more than 92%. The chromosomal localization of the human SDF-2 gene was mapped to 17q11.2. The aa sequence of SDF-2 shows similarity to those of yeast dolichyl phosphate-d-mannose:protein mannosyltransferases, Pmtlp [Strahl-Bolsinger et al. (1993) Proc. Natl. Acad. Sci. USA 90, 8164-8168] and Pmt2p [Lussier et al. (1995) J. Biol. Chem. 270, 2770-2775], whose activities have not been detected in higher eukaryotes.
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