Chemokine requirements for B cell entry to lymph nodes and Peyer's patches
T Okada, VN Ngo, EH Ekland, R Förster… - The Journal of …, 2002 - rupress.org
T Okada, VN Ngo, EH Ekland, R Förster, M Lipp, DR Littman, JG Cyster
The Journal of experimental medicine, 2002•rupress.orgB cell entry to lymph nodes and Peyer's patches depends on chemokine receptor signaling,
but the principal chemokine involved has not been defined. Here we show that the homing
of CXCR4−/− B cells is suppressed in CCL19 (ELC)-and CCL21 (SLC)-deficient paucity of
lymph node T cells mice, but not in wild-type mice. We also find that CXCR4 can contribute
to T cell homing. Using intravital microscopy, we find that B cell adhesion to high endothelial
venules (HEVs) is disrupted when CCR7 and CXCR4 are predesensitized. In Peyer's …
but the principal chemokine involved has not been defined. Here we show that the homing
of CXCR4−/− B cells is suppressed in CCL19 (ELC)-and CCL21 (SLC)-deficient paucity of
lymph node T cells mice, but not in wild-type mice. We also find that CXCR4 can contribute
to T cell homing. Using intravital microscopy, we find that B cell adhesion to high endothelial
venules (HEVs) is disrupted when CCR7 and CXCR4 are predesensitized. In Peyer's …
B cell entry to lymph nodes and Peyer's patches depends on chemokine receptor signaling, but the principal chemokine involved has not been defined. Here we show that the homing of CXCR4−/− B cells is suppressed in CCL19 (ELC)- and CCL21 (SLC)-deficient paucity of lymph node T cells mice, but not in wild-type mice. We also find that CXCR4 can contribute to T cell homing. Using intravital microscopy, we find that B cell adhesion to high endothelial venules (HEVs) is disrupted when CCR7 and CXCR4 are predesensitized. In Peyer's patches, B cell entry is dependent on CXCR5 in addition to CCR7/CXCR4. CXCL12 (SDF1) is displayed broadly on HEVs, whereas CXCL13 (BLC) is found selectively on Peyer's patch follicular HEVs. These findings establish the principal chemokine and chemokine receptor requirements for B cell entry to lymph nodes and Peyer's patches.
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