Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution
AB Lyons - Journal of immunological methods, 2000 - Elsevier
Since its introduction in 1994 (J. Immunol. Methods 171 (1994) 131), the flow cytometric
analysis of lymphocyte proliferation by serial halving of the fluorescence intensity of the vital
dye CFSE (carboxyfluorescein diacetate, succinimidyl ester or CFDA-SE) has become
widely used in immunological laboratories around the world. This technique allows the
visualisation of eight to 10 discrete cycles of cell division by flow cytometry, both in vitro and
in vivo. Appropriately conjugated antibodies can be used to probe surface marker changes …
analysis of lymphocyte proliferation by serial halving of the fluorescence intensity of the vital
dye CFSE (carboxyfluorescein diacetate, succinimidyl ester or CFDA-SE) has become
widely used in immunological laboratories around the world. This technique allows the
visualisation of eight to 10 discrete cycles of cell division by flow cytometry, both in vitro and
in vivo. Appropriately conjugated antibodies can be used to probe surface marker changes …