Subnucleosome structures as substrates in enzyme-linked immunosorbent assays

RW Burlingame, RL Rubin - Journal of immunological methods, 1990 - Elsevier
Histone preparations preserving the tertiary and quaternary structure of histone-histone
complexes and histone-DNA complexes, as well as individual histones, were isolated or
reconstituted. Various parameters were tested in order to determine the suitability of these
complexes for use as substrates in enzyme-linked immunosorbent assays. The protein
concentration required to saturate the solid phase was determined, and the amount of
bound protein was quantified by the micro-bicinchoninic acid protein assay. In addition, the …