[HTML][HTML] Osteopontin transcription in aortic vascular smooth muscle cells is controlled by glucose-regulated upstream stimulatory factor and activator protein-1 activities
M Bidder, JS Shao, N Charlton-Kachigian… - Journal of Biological …, 2002 - ASBMB
The expression of the matrix cytokine osteopontin (OPN) is up-regulated in aortic vascular
smooth muscle cells (VSMCs) by diabetes. OPN expression in cultured VSMCs is
reciprocally regulated by glucose and 2-deoxyglucose (2-DG; inhibitor of cellular glucose
metabolism). Systematic analyses of OPNpromoter-luciferase reporter constructs identify a
CCTCATGAC motif at nucleotides− 80 to− 72 relative to the initiation site that supports OPN
transcription in VSMCs. The region− 83 to− 45 encompassing this motif confers basal and …
smooth muscle cells (VSMCs) by diabetes. OPN expression in cultured VSMCs is
reciprocally regulated by glucose and 2-deoxyglucose (2-DG; inhibitor of cellular glucose
metabolism). Systematic analyses of OPNpromoter-luciferase reporter constructs identify a
CCTCATGAC motif at nucleotides− 80 to− 72 relative to the initiation site that supports OPN
transcription in VSMCs. The region− 83 to− 45 encompassing this motif confers basal and …