Optimal conditions for proliferation of bone marrow‐derived mouse macrophages in culture: The roles of CSF‐1, serum, Ca2+, and adherence
DA Hume, S Gordon - Journal of cellular physiology, 1983 - Wiley Online Library
DA Hume, S Gordon
Journal of cellular physiology, 1983•Wiley Online LibraryA method is described for the analysis of [3H]‐thymidine incorporation in microtitre cultures
of bone marrow‐derived mouse macrophage responding to macrophage colony‐stimulating
factor (CSF‐1).[3H]‐thymidine incorporation depends on cell density, culture medium, and
the concentration of CSF‐1 and serum, but is independent of Ca2+. Bone marrow‐derived
macrophages are strongly adherent, but adherence can be dissociated from [3H]‐thymidine
incorporation.
of bone marrow‐derived mouse macrophage responding to macrophage colony‐stimulating
factor (CSF‐1).[3H]‐thymidine incorporation depends on cell density, culture medium, and
the concentration of CSF‐1 and serum, but is independent of Ca2+. Bone marrow‐derived
macrophages are strongly adherent, but adherence can be dissociated from [3H]‐thymidine
incorporation.
Abstract
A method is described for the analysis of [3H]‐thymidine incorporation in microtitre cultures of bone marrow‐derived mouse macrophage responding to macrophage colony‐stimulating factor (CSF‐1). [3H]‐thymidine incorporation depends on cell density, culture medium, and the concentration of CSF‐1 and serum, but is independent of Ca2+. Bone marrow‐derived macrophages are strongly adherent, but adherence can be dissociated from [3H]‐thymidine incorporation.
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