Material on the surface of activated T-cells was displaced following incubation with a
sulfated polysaccharide, dextrin 2-sulfate (D2S), and purified by anion-exchange
chromatography. This revealed a complex comprising histones H2A, H2B, H3, and H4 and
DNA fragmented into 180-base pair units characteristic of mono-, di-, tri, and
polynucleosomes, a pattern of fragmentation similar to that found in apoptotic cells. An
antibody raised against the purified nucleosome preparation bound to the plasma …