[HTML][HTML] Insulin stimulates PKCζ-mediated phosphorylation of insulin receptor substrate-1 (IRS-1): a self-attenuated mechanism to negatively regulate the function of …
YF Liu, K Paz, A Herschkovitz, A Alt… - Journal of Biological …, 2001 - ASBMB
Incubation of rat hepatoma Fao cells with insulin leads to a transient rise in Tyr
phosphorylation of insulin receptor substrate (IRS) proteins. This is followed by elevation in
their P-Ser/Thr content, and their dissociation from the insulin receptor (IR). Wortmannin, a
phosphatidylinositol 3-kinase (PI3K) inhibitor, abolished the increase in the P-Ser/Thr
content of IRS-1, its dissociation from the IR, and the decrease in its P-Tyr content following
60 min of insulin treatment, indicating that the Ser kinases that negatively regulate IRS-1 …
phosphorylation of insulin receptor substrate (IRS) proteins. This is followed by elevation in
their P-Ser/Thr content, and their dissociation from the insulin receptor (IR). Wortmannin, a
phosphatidylinositol 3-kinase (PI3K) inhibitor, abolished the increase in the P-Ser/Thr
content of IRS-1, its dissociation from the IR, and the decrease in its P-Tyr content following
60 min of insulin treatment, indicating that the Ser kinases that negatively regulate IRS-1 …