[PDF][PDF] Induction of rapid T cell activation and tolerance by systemic presentation of an orally administered antigen
Immunity, 1998•cell.com
To understand how orally introduced antigen regulates peripheral immune responses, we
fed cytochrome c protein to mice transgenic for the β chain of a cytochrome c–specific TCR
and followed the antigen-specific T cell responses with a cyt c/IE k tetramer staining reagent.
We find that within 6 hr of cytochrome c administration, antigen-specific systemic T cell
activation is induced, and spleen cells gain the ability to stimulate cytochrome c–specific T
cell responses. Feeding multiple low doses of cytochrome c down-regulates the systemic …
fed cytochrome c protein to mice transgenic for the β chain of a cytochrome c–specific TCR
and followed the antigen-specific T cell responses with a cyt c/IE k tetramer staining reagent.
We find that within 6 hr of cytochrome c administration, antigen-specific systemic T cell
activation is induced, and spleen cells gain the ability to stimulate cytochrome c–specific T
cell responses. Feeding multiple low doses of cytochrome c down-regulates the systemic …
Abstract
To understand how orally introduced antigen regulates peripheral immune responses, we fed cytochrome c protein to mice transgenic for the β chain of a cytochrome c–specific TCR and followed the antigen-specific T cell responses with a cyt c/I-Ek tetramer staining reagent. We find that within 6 hr of cytochrome c administration, antigen-specific systemic T cell activation is induced, and spleen cells gain the ability to stimulate cytochrome c–specific T cell responses. Feeding multiple low doses of cytochrome c down-regulates the systemic immune response, which can be correlated with a reduction of antigen-specific T cells and not with immune deviation. These results suggest that systemic distribution of antigen contributes significantly to oral tolerance induction.
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