[PDF][PDF] Mixed bone marrow chimerism as a treatment for autoimmune diabetes
I Leykin, B Nikolic, M Sykes - Transplantation proceedings, 2001 - academia.edu
I Leykin, B Nikolic, M Sykes
Transplantation proceedings, 2001•academia.eduMETHODS Depleting dose of anti-CD4, anti-CD8, and anti-Thy1, 2, as well as anti-CD40L
mAbs were given to diabetic or nondiabetic NOD mice (H-2g7), which then received 4 Gy of
whole-body irradiation, followed by BMT from allogeneic C57BL/6 mice (H-2b). Levels of
donor chimerism in peripheral blood, spleen, bone marrow, and thymi were measured by
FACS. Tolerance was assessed by skin grafting, MLR, and CML. Either syngeneic or
allogeneic pancreatic islets were transplanted under the kidney capsule of diabetic mice …
mAbs were given to diabetic or nondiabetic NOD mice (H-2g7), which then received 4 Gy of
whole-body irradiation, followed by BMT from allogeneic C57BL/6 mice (H-2b). Levels of
donor chimerism in peripheral blood, spleen, bone marrow, and thymi were measured by
FACS. Tolerance was assessed by skin grafting, MLR, and CML. Either syngeneic or
allogeneic pancreatic islets were transplanted under the kidney capsule of diabetic mice …
METHODS
Depleting dose of anti-CD4, anti-CD8, and anti-Thy1, 2, as well as anti-CD40L mAbs were given to diabetic or nondiabetic NOD mice (H-2g7), which then received 4 Gy of whole-body irradiation, followed by BMT from allogeneic C57BL/6 mice (H-2b). Levels of donor chimerism in peripheral blood, spleen, bone marrow, and thymi were measured by FACS. Tolerance was assessed by skin grafting, MLR, and CML. Either syngeneic or allogeneic pancreatic islets were transplanted under the kidney capsule of diabetic mice receiving conditioning with or without BMT.
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