Gene correction of the apolipoprotein (Apo) E2 phenotype to wild-type ApoE3 by in situ chimeraplasty
AD Tagalakis, IR Graham, DR Riddell… - Journal of Biological …, 2001 - ASBMB
Apolipoprotein (apo) E is a polymorphic plasma protein, synthesized mainly by liver. Here,
we evaluate whether synthetic DNA-RNA oligonucleotides (chimeraplasts) can convert a
dysfunctional isoform, apoE2 (C→ T, R158C), which causes Type III hyperlipidemia and
premature atherosclerosis, into apoE3. First, we treated recombinant Chinese hamster ovary
cells stably secreting apoE2 with a 68-mer apoE2 to apoE3 chimeraplast. About one-third of
apoE2 was converted to apoE3, and the repair was stable through 12 passages. Subcloning …
we evaluate whether synthetic DNA-RNA oligonucleotides (chimeraplasts) can convert a
dysfunctional isoform, apoE2 (C→ T, R158C), which causes Type III hyperlipidemia and
premature atherosclerosis, into apoE3. First, we treated recombinant Chinese hamster ovary
cells stably secreting apoE2 with a 68-mer apoE2 to apoE3 chimeraplast. About one-third of
apoE2 was converted to apoE3, and the repair was stable through 12 passages. Subcloning …
