Notch is a large cell-surface receptor known to be an essential player in a wide variety of
developmental cascades. Here we show that Notch1 endogenously expressed in Chinese
hamster ovary cells is modified with O-linked fucose andO-linked glucose saccharides, two
unusual forms ofO-linked glycosylation found on epidermal growth factor-like (EGF)
modules. Interestingly, both modifications occur as monosaccharide and oligosaccharide
species. Through exoglycosidase digestions we determined that the O-linked fucose …