Distal tubular segments of the rabbit kidney after adaptation to altered Na-and K-intake: I. Structural changes
B Kaissling, M Le Hir - Cell and tissue research, 1982 - Springer
B Kaissling, M Le Hir
Cell and tissue research, 1982•SpringerThe baso-lateral cell-membrane area in kidney tubules appears to be associated with the
capacity for electrolyte transport; in the rabbit, it decreases from the distal convoluted tubule
(DCT-cells) over the connecting tubule (CNT-cells) to the cortical collecting duct (principal
cells). Adaptation to low Na-, high K-intake changes this pattern: CNT-cells at the beginning
of the connecting tubule have the highest membrane area, which decreases along the
segment, but remains two-fold higher than in controls. Principal cells have a four-fold higher …
capacity for electrolyte transport; in the rabbit, it decreases from the distal convoluted tubule
(DCT-cells) over the connecting tubule (CNT-cells) to the cortical collecting duct (principal
cells). Adaptation to low Na-, high K-intake changes this pattern: CNT-cells at the beginning
of the connecting tubule have the highest membrane area, which decreases along the
segment, but remains two-fold higher than in controls. Principal cells have a four-fold higher …
Summary
The baso-lateral cell-membrane area in kidney tubules appears to be associated with the capacity for electrolyte transport; in the rabbit, it decreases from the distal convoluted tubule (DCT-cells) over the connecting tubule (CNT-cells) to the cortical collecting duct (principal cells).
Adaptation to low Na-, high K-intake changes this pattern: CNT-cells at the beginning of the connecting tubule have the highest membrane area, which decreases along the segment, but remains two-fold higher than in controls. Principal cells have a four-fold higher membrane area than in controls. Simultaneous treatment with the antimineralocorticoid canrenoate-K inhibits the structural changes in CNT-cells only in end-portions of the connecting tubule and in principal cells.
After prolonged high Na-, low K-intake DCT-cells display a two-fold higher membrane area than controls, while CNT-cells and principal cells are not affected. Simultaneous treatment with DOCA does not affect the DCT-cells but provokes a moderate increase in membrane area in CNT-cells, and a 5.5-fold increase in principal cells.
The data provide evidence that DCT-, CNT- and principal cells are functionally different cell types. The baso-lateral cell-membrane area, associated with electrolyte-transport capacity, appears to be influenced in DCT-cells mainly by Na-intake, in CNT-cells mainly by K-intake and in part also by mineralocorticoids, and in principal cells mainly by mineralocorticoids.
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