Expression of cripto, a Novel Gene of the Epidermal Growth Factor Gene Family, Leads to in Vitro Transformation of a Normal Mouse Mammary Epithelial Cell Line
Cancer research, 1991•AACR
Abstract cripto is a gene encoding an epidermal growth factor-related protein that is
expressed in undifferentiated embryonal carcinoma cells. To ascertain if cripto is capable of
functioning as a transforming gene, a full-length human cripto complementary DNA under
the transcriptional control of the Rous sarcoma virus long terminal repeat has been
cotransfected with the selectable pSV2 neo marker plasmid into immortalized mouse NOG-8
mammary epithelial cells. Several neomycin-resistant clones were isolated that express high …
expressed in undifferentiated embryonal carcinoma cells. To ascertain if cripto is capable of
functioning as a transforming gene, a full-length human cripto complementary DNA under
the transcriptional control of the Rous sarcoma virus long terminal repeat has been
cotransfected with the selectable pSV2 neo marker plasmid into immortalized mouse NOG-8
mammary epithelial cells. Several neomycin-resistant clones were isolated that express high …
Abstract
cripto is a gene encoding an epidermal growth factor-related protein that is expressed in undifferentiated embryonal carcinoma cells. To ascertain if cripto is capable of functioning as a transforming gene, a full-length human cripto complementary DNA under the transcriptional control of the Rous sarcoma virus long terminal repeat has been cotransfected with the selectable pSV2neo marker plasmid into immortalized mouse NOG-8 mammary epithelial cells. Several neomycin-resistant clones were isolated that express high levels of a specific cripto 4.5-kilobase mRNA transcript and possess multiple copies of cripto plasmid DNA. NOG-8 cells that express cripto are able to clone in soft agar and exhibit an approximately 3-fold increase in their anchorage-dependent growth in serum-free medium as compared to the neo-transfected NOG-8 cells. However, none of the cripto-expressing NOG-8 clones are able to form tumors in nude mice.
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