Synthesis of Triple Helix Forming Oligonucleotides Containing 2′-Deoxyformycin A
TS Rao, ME Hogan, GR Revankar - Nucleosides, Nucleotides & …, 1994 - Taylor & Francis
TS Rao, ME Hogan, GR Revankar
Nucleosides, Nucleotides & Nucleic Acids, 1994•Taylor & FrancisAbstract N7-Benzoyl-2′-deoxyformycin A (5) was prepared from formycin A and
incorporated into the triple helix forming oligonucleotide PRE2ap at CG inversion sites. The
modified oligonucleotide containing three substitutions of 2′-deoxyformycin A displayed a
10-fold increase in binding affinity as compared to its unmodified counterpart. This provided
a method to accommodate CG inversion sites within target sites for antiparallel triple helix
formation.
incorporated into the triple helix forming oligonucleotide PRE2ap at CG inversion sites. The
modified oligonucleotide containing three substitutions of 2′-deoxyformycin A displayed a
10-fold increase in binding affinity as compared to its unmodified counterpart. This provided
a method to accommodate CG inversion sites within target sites for antiparallel triple helix
formation.
Abstract
N7-Benzoyl-2′-deoxyformycin A (5) was prepared from formycin A and incorporated into the triple helix forming oligonucleotide PRE2ap at CG inversion sites. The modified oligonucleotide containing three substitutions of 2′-deoxyformycin A displayed a 10-fold increase in binding affinity as compared to its unmodified counterpart. This provided a method to accommodate CG inversion sites within target sites for antiparallel triple helix formation.
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